Special stains in histopathology pdf

Histology Main Menu. These manuals are in Adobe Acrobat PDF format and require the Acrobat Reader for viewing. Staining Manuals Fixative Solutions: Each of the following .PDF files contains the procedure plus a sample container label. Each of the following .PDF files contains the procedure for a special stain, a procedure card, and Anatomic Pathology Special Stains Group I for Microorganisms Special Stains Group I 88312 Primary Demonstration of: Fite stain Stains for mycobacteria leprae Giemsa (h. pylori) Helicobacter Gomori's methenamine silver (GMS) Fungus, Pneumocystis, Bacteria Gram Gram-positive and gram-negative bacteria Gridley Fungus Steiner Spirochetes, Bacteria Generally, when special staining is performed on a section of tissue, a positive control section is also stained. This involves simultaneous staining of a section of tissue known to contain the substance in question. This will be given to the pathologist or researcher at the same time as the test tissue section, and serves various purposes. Additional special stains may be used to highlight or identify features that are not easily seen on an H&E stain. The choice of stain or panel of stains depends on the findings on initial assessment, the clinical context, and the preference of the pathologist. A special stain is a staining technique to highlight. Special Stains Special Stains in Histology Mucin stains There are a variety of mucin stains, all attempting to demonstrate one or more types of mucopolysaccharide substances in tissues. The types of mucopolysaccharides are as follows: • Neutral - These can be found in glands of the GI Chemical Analysis, Life Sciences, and Diagnostics | Agilent common and routine stain used in histopathology is a combination of haematoxylin and eosin (H&E). However, it simply cannot answer all the questions that special stains are synthetic chemicals and many of the risks in a laboratory will relate to chemical hazards. Each chemical, therefore, needs to be considered carefully while using [7]. See procedure and table below for proper handling, collection, and submission of surgical pathology specimens. II. PROCEDURES 1. Consider the need to notify the Airport Trade Center Laboratory at (808) 834- 5128 in advance of any tests requiring special handling. (see below) 2. Use universal precautions in handling all tissue specimens. 3. Counterstains and special stains for immunohistochemistry. Histology stains, fluorescent stains and special stains are used to stain specific cell and tissue structures, and also to stain microorganisms to help identify the location of primary antibody staining. Find information below about. This page is part of our IHC application guide A counter stain is the application to the original stain, usually nuclear, or one or more dyes that by contrast will bring out heavy counterstain is to be avoided least it mask the nuclear stain. It can be done either by using dilute stain or cutting down the staining time. Some counterstains which are acidic may lighten or remove the nuclear PAS (periodic acid-Schiff) This an all-around useful stain for many things. It stains glycogen, mucin, mucoprotein, glycoprotein, as well as fungi. A predigestion step with amylase will remove staining for glycogen. PAS is useful for outlining tissue structures--basement membranes, capsules, blood vessels, etc. What is staining in histopathology. What is special staining techniq