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To understand real-time PCR it is easier to begin with the principles of a basic PCR: PCR is a technique for amplifying DNA. There are 2 reasons why.1.1 Key Concepts of Real-Time PCR. 2. 1.1.1 What Is Real-Time PCR? 2. 1.1.2 How Real-Time PCR Works. 3. 1.1.3 Hallmarks of an Optimized qPCR Assay. tative PCR (qPCR). Numerous qPCR detection chemistries and instruments are now available to answer a wide range of questions. For instance, qPCR can be used The principle, and aim, of the PCR technology is to specifically increase a target from an undetectable amount of starting material. In classical PCR, at the By plotting fluorescence against the cycle number, the real-time PCR instrument generates an amplification plot that represents the accumulation of product over The basic principle used in the analysis of real-time PCR data is that the number of cycles necessary to reach a fixed concentration of amplicon in the reaction Quantitative Real-Time PCR. Detection of PCR product growth throughout the amplification process. •No post-PCR processing required.