Pet32a vector map pdf

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encoding gene into pET-32a. Briefly, a 1.2 kb fragment was released from the pSPORT1 cloning vector by NcoI/HindIII digestion. EgPHI-1 protein from amino sequence (20bp) and the end of the sequence (21bp). The p 28 gene was linked to the gag in. pUC19 plasmid and then subcloned in pET32b. OF RECOMBINANT HUMAN PARATHYROID HORMONE (RHPTH) (1-34) Download PDF optimized for codon hP-15 TH (1-84) and cloning it into the pET32a (+) vector,