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This manual is the original documentation for the OPUS spectroscopic software. OPUS Reference Manual. Bruker Optik GmbH. 8.2. Spectrum Subtraction. Downloads related to the Bruker OPUS software for state-of-the-art measurement, processing and evaluation of FTIR, NIR and Raman Spectra. OPUS Reference Manual. Bruker Optik GmbH. 8.2. Spectrum Subtraction. This kind of calculation subtracts spectra, with one multiplication factor each,. For more detailed information on the user account system refer to the OPUS Reference Manual. 4. Click the Login button. The About OPUS dialog opens. spectrum is a good candidate for baseline correction (automatic or manual). Page 29. Bruker Tensor 27 FT-IR Instructions (V1.1). P a g e | This manual is the original documentation for the OPUS spectroscopic software. Bruker Optik GmbH. OPUS Reference Manual. 259. Chapt. 8. 8.2. 8.2.2 TriBox Mini D01-049en201706 OPUS Manual OPUS is a spectral sensor for the online measurement of nitrogen and carbon compounds.